研究方向
Mouse Klotho 是一种抗衰老蛋白，在调节衰老、代谢和多种疾病中发挥重要作用。它最初被发现与衰老相关，因其缺失会导致小鼠出现早衰表型。以下是关于Mouse Klotho的详细信息：
Mouse Klotho是一种重要的抗衰老蛋白，在代谢调控、衰老和疾病中发挥关键作用。其在衰老相关疾病中的潜在治疗价值使其成为研究热点。
生物活性
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human FGF RIIIc. The ED50 forthis effect is 0.04- 0.4 µg/mL in the presence of 1 µg/mL of Recombinant Human FGF‑23 and 10 µg/mL of heparin.
Swiss Prot:
O35082-1
种属:
Mouse
表达宿主:
293F
表达区间:
Ala35-Lys982
实际分子量:
125- 130 kDa
标签:
C-His
纯度：
>90% by SDS-PAGE.
内毒素：
＜0.1EU/ug of the protein by LAL method.
制剂：
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述：
Recombinant Mouse Mouse Klotho Protein is produced by 293F expression system. The target protein is expressed with sequence (Ala35-Lys982) of Mouse Mouse Klotho (Accession O35082-1) fused with a C-His.
储存：
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶：
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻
