研究方向
Mouse Osteoprotegerin（OPG）是一种分泌型糖蛋白，属于肿瘤坏死因子受体超家族（TNFRSF）。它在骨代谢和免疫调节中发挥关键作用，尤其是在抑制破骨细胞分化和骨吸收中。以下是关于Mouse OPG的详细信息：
Mouse Osteoprotegerin（OPG）是一种重要的骨代谢调节蛋白，通过抑制破骨细胞的分化和活性，在维持骨稳态和免疫调节中发挥关键作用。其在骨质疏松症和肿瘤骨转移中的潜在治疗价值使其成为研究热点。
生物活性
Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L‑929 mouse fibroblast cells treated with TRAIL. The ED50 forthis effect is 3-15 ng/mL in the presence of 12 ng/mL of cross-linked Recombinant Human TRAIL/TNFSF10.
Swiss Prot:
O08712
生物活性:
有
种属:
Mouse
表达宿主:
293F
表达区间:
Glu22-Leu401 (Arg138GIn)
实际分子量:
85-95 kDa
标签:
C-hFc&His tag
纯度：
>95% by SDS-PAGE.
内毒素：
＜0.1EU/ug of the protein by LAL method.
制剂：
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述：
Recombinant Mouse Mouse Osteoprotegerin Protein is produced by 293F expression system. The target protein is expressed with sequence (Glu22-Leu401 (Arg138GIn)) of Mouse Mouse Osteoprotegerin (Accession O08712) fused with a C-hFc&His tag.
储存：
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶：
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
