研究方向
Mouse Semaphorin 3A（SEMA3A）是一种分泌型信号蛋白，属于Semaphorin蛋白家族。它在神经系统的发育、血管生成和免疫调节中发挥重要作用。以下是关于Mouse SEMA3A的详细信息：
Mouse Semaphorin 3A（SEMA3A）是一种多功能信号蛋白，在神经系统发育、血管生成和免疫调节中发挥关键作用。其研究不仅有助于理解生物学的基本机制，也为开发新的治疗策略提供了潜在靶点。
Measured by its ability to cause collapse of chick embryonic dorsalroot ganglia (DRG) neuron growth cones. 20-60 ng/mL of Recombinant Mouse Semaphorin 3A Fc Chimera causes >50% growth cone collapse in the presence 10 ng/mL Recombinant Human beta ‑NGF.
Swiss Prot:
O08665
生物活性:
有
种属:
Mouse
表达宿主:
293F
表达区间:
Asn21-Lys747: Arg552Ala, Arg554Ala, Arg555Ala, Arg618Ala, Lys619Ala, Lys642Ala, Lys643Ala
实际分子量:
115- 120 kDa
标签:
C-hFc&His tag
纯度：
>90% by SDS-PAGE.
内毒素：
＜0.1EU/ug of the protein by LAL method.
制剂：
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述：
Recombinant Mouse Mouse Semaphorin 3A Protein is produced by 293F expression system. The target protein is expressed with sequence (Asn21-Lys747: Arg552Ala, Arg554Ala, Arg555Ala, Arg618Ala, Lys619Ala, Lys642Ala, Lys643Ala) of Mouse Mouse Semaphorin 3A (Accession O08665) fused with a C-hFc&His tag.
储存：
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶：
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
