研究方向
Human Carboxylesterase 2(CES2)是一种重要的水解酶,属于羧酸酯酶家族。它在药物代谢、脂质代谢和解毒过程中发挥关键作用。以下是关于CES2的详细信息:
Human Carboxylesterase 2(CES2)是一种重要的水解酶,在药物代谢、脂质代谢和解毒过程中发挥关键作用。其研究不仅有助于理解代谢生物学的基本机制,也为开发新的治疗策略提供了潜在靶点。
Measured by its ability to hydrolyze pnitrophenylacetate. The specific activity is >30,000 pmol/min/μg, measured under the described conditions.
Swiss Prot:
O00748
生物活性:
有
种属:
Human
表达宿主:
293F
表达区间:
Gln27-Leu559
实际分子量:
63 kDa
标签:
C- 10*His tag
纯度:
>95% by SDS-PAGE.
内毒素:
<0.1EU/ug of the protein by LAL method.
制剂:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述:
Recombinant Human Human Carboxylesterase 2/CES2 Protein is produced by 293F expression system. The target protein is expressed with sequence (Gln27-Leu559) of Human Human Carboxylesterase 2/CES2 (Accession O00748) fused with a C- 10*His tag.
储存:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
复溶:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。