研究方向
Human Frizzled-9(FZD9)是Wnt信号通路中的一种重要受体,属于Frizzled家族。它在细胞发育、组织稳态和疾病发生中起关键作用。以下是关于Human Frizzled-9的详细介绍:
Human Frizzled-9在细胞发育、组织稳态和疾病发生中具有重要作用,其独特的结构和功能使其成为生物医学研究中的热点。未来,FZD9可能在疾病诊断和治疗中发挥重要价值。
1. 基本结构与功能
2. 表达与分布
3. 生物学作用
4. 临床意义
5. 研究进展
总结
Measured by its ability to bind biotinylated recombinant mouse Wnt-3a in a functional ELISA with an ED50 of 15-90 ng/mL.
Swiss Prot:
O00144
生物活性:
有
种属:
Human
表达宿主:
293F
表达区间:
Leu23-Asp185
实际分子量:
54-63 kDa
标签:
C-hFc&His tag
纯度:
>90% by SDS-PAGE.
内毒素:
<0.1EU/ug of the protein by LAL method.
制剂:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述:
Recombinant Human Human Frizzled-9 Protein is produced by 293F expression system. The target protein is expressed with sequence (Leu23-Asp185) of Human Human Frizzled-9 (Accession O00144) fused with a C-hFc&His tag.
储存:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
复溶:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。