研究方向
Mouse Semaphorin 3B（Sema3B）是一种分泌型蛋白，属于Semaphorin家族，在神经发育、血管生成和肿瘤抑制中起重要作用。以下是关于Mouse Semaphorin 3B的详细介绍：
Mouse Semaphorin 3B在神经发育、血管生成和肿瘤抑制中具有重要作用，其独特的结构和功能使其成为神经科学和肿瘤学研究中的热点。未来，Sema3B可能在疾病诊断和治疗中发挥重要价值。
Measured by its ability to inhibit proliferation of the A549 human lung carcinoma cells. The ED50 for this effect is 100-400 ng/mL.
Swiss Prot:
M9MMK0
生物活性:
有
种属:
Mouse
表达宿主:
293F
表达区间:
Pro26 - Asp722: Arg551Ala, Arg553Ala, Arg554Ala, Arg635Ala, Arg636Ala
实际分子量:
104-115 kDa
标签:
C-hFc&His tag
纯度：
>90% by SDS-PAGE.
内毒素：
＜0.1EU/ug of the protein by LAL method.
制剂：
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述：
Recombinant Mouse Mouse Semaphorin 3B Protein is produced by 293F expression system. The target protein is expressed with sequence (Pro26 - Asp722: Arg551Ala, Arg553Ala, Arg554Ala, Arg635Ala, Arg636Ala) of Mouse Mouse Semaphorin 3B (Accession M9MMK0) fused with a C-hFc&His tag.
储存：
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶：
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
