研究方向
Mouse MAdCAM-1（Mucosal Addressin Cell Adhesion Molecule-1，黏膜地址素细胞黏附分子1）是一种在黏膜免疫系统中起关键作用的细胞黏附分子。以下是关于Mouse MAdCAM-1的详细介绍：
Mouse MAdCAM-1在黏膜免疫和炎症反应中具有重要作用，其独特的结构和功能使其成为免疫学和疾病研究中的热点。未来，MAdCAM-1可能在疾病诊断和治疗中发挥重要价值。
Measured by the ability of the immobilized protein to support the adhesion of HuT 78 human cutaneous T cell lymphoma cells. When 5 x 104 cells/well are added to mouse MAdCAM-1/Fc Chimera coated plates (10 µg/mL with 100 µL/well), approximately 80- 90% will adhere after MnCl2 stimulation for 1 hourincubation at room temperature.
Swiss Prot:
G5E838
生物活性:
有
种属:
Mouse
表达宿主:
293F
表达区间:
Gln22 - Thr365
实际分子量:
55-60 kDa
标签:
C-His
纯度：
>95% by SDS-PAGE.
内毒素：
＜0.1EU/ug of the protein by LAL method.
制剂：
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述：
Recombinant Mouse Mouse MAdCAM-1 Protein is produced by 293F expression system. The target protein is expressed with sequence (Gln22 - Thr365) of Mouse Mouse MAdCAM-1 (Accession G5E838) fused with a C-His.
储存：
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶：
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
