研究方向
Human RGM-B（Repulsive Guidance Molecule B，排斥性导向分子B）是一种在神经发育和免疫调节中起重要作用的蛋白质。它属于RGM家族，与神经系统的轴突导向、细胞迁移以及免疫系统的功能密切相关。以下是关于Human RGM-B的详细介绍：
Human RGM-B在神经发育和免疫调节中具有重要作用，其独特的结构和功能使其成为神经科学和免疫学研究中的热点。未来，RGM-B可能在疾病诊断和治疗中发挥重要价值。
Measured by its binding ability in a functional ELISA. Immoblized rhBMP-4 at 1 µg/mL (100 µL/well) can bind rhRGM-B with a linear range of 10-500 ng/mL
Swiss Prot:
AAH67736
生物活性:
有
种属:
Human
表达宿主:
293F
表达区间:
Gly87-Asp209 (N-terminus chain) & Pro210-Ser452 (C-terminus chain)
实际分子量:
20kDa & 35 kDa
标签:
C-His
纯度：
>95% by SDS-PAGE.
内毒素：
＜0.1EU/ug of the protein by LAL method.
制剂：
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述：
Recombinant Human Human RGM-B Protein is produced by 293F expression system. The target protein is expressed with sequence (Gly87-Asp209 (N-terminus chain) & Pro210-Ser452 (C-terminus chain)) of Human Human RGM-B (Accession AAH67736) fused with a C-His.
储存：
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶：
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
