研究方向
Human CD142（也称为组织因子，Tissue Factor，TF）是一种跨膜糖蛋白，在凝血和炎症过程中起关键作用。以下是关于Human CD142的详细介绍：
1. 基本结构与功能
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结构：CD142是一种单链跨膜蛋白，其胞外部分包含一个凝血因子VII结合位点。
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功能：作为凝血级联反应的关键启动因子，CD142通过与凝血因子VII/VIIa结合，激活外源性凝血途径，促进血栓形成。
2. 表达与分布
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表达细胞：CD142主要在内皮细胞、单核细胞和某些肿瘤细胞中表达。
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组织分布：广泛分布于血管壁、胎盘和脑组织等部位。
3. 生物学作用
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凝血功能：CD142在血管损伤时暴露于血液中，启动凝血过程，防止出血。
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炎症调节：CD142通过调控细胞因子和趋化因子的释放，参与炎症反应。
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肿瘤进展：在某些肿瘤中，CD142的高表达与肿瘤生长、转移和血管生成密切相关。
4. 临床意义
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疾病关联：CD142的表达异常与多种疾病相关，包括血栓性疾病、癌症和败血症。
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治疗靶点：CD142是抗凝治疗和肿瘤治疗的重要靶点，已有多种针对CD142的药物在研发或临床试验中。
5. 研究进展
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基础研究：目前的研究主要集中在CD142的信号传导机制及其在疾病中的作用。
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应用研究：探索基于CD142的治疗策略，如开发特异性抗体或小分子抑制剂，以调控凝血和炎症反应。
总结
Human CD142在凝血、炎症和肿瘤进展中具有重要作用，其独特的结构和功能使其成为医学研究中的热点。未来，CD142可能在疾病诊断和治疗中发挥重要价值。
生物活性
Measured by its ability to activate Coagulation Factor VII in cleaving a fluorogenic peptide substrate Boc-VPR-AMC. The AC50 is <24.0 μg/mL.
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述
Recombinant Human Coagulation factor III/Tissue factor/CD142 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly34-Glu251) of human Coagulation Factor III/Tissue Factor/CD142 (Accession #NP_001984.1) fused with a 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
背景信息
The protein is coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.
别名
CD142; TF; TFA;F3;TF;TFA
