活性重组蛋白 Recombinant Human TIM 3 / HAVCR2 / CD366 / KIM-3 / SPTCL Protein

T 细胞免疫球蛋白 mucin-3 (TIM-3) 属于 Ig 超家族。TIM-3 通常由多种小鼠和人类免疫细胞类型表达。TIM-3 首次在产生 IFN-γ 的 Th1 和 Tc1 细胞上发现。TIM-3 作为抑制性受体，抑制 T 细胞功能。TIM-3 与自身免疫和癌症中免疫反应的调节有关。

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商品分类：重组蛋白　　种属： Human

商品编号： GX004927　　库存： 100支

上市时间： 2024-12-11 16:51:20　　浏览次数： 760次

研究方向

T 细胞免疫球蛋白 mucin-3 (TIM-3) 属于 Ig 超家族。TIM-3 通常由多种小鼠和人类免疫细胞类型表达。TIM-3 首次在产生 IFN-γ 的 Th1 和 Tc1 细胞上发现。TIM-3 作为抑制性受体，抑制 T 细胞功能。TIM-3 与自身免疫和癌症中免疫反应的调节有关。
TIM-3 具有多种不同的配体：半乳糖凝集素 9、磷脂酰丝氨酸 (PtdSer)、CEACAM1 和 HMGB1，这些配体与 TIM3 胞外免疫球蛋白 V 结构域上的不同区域结合。TIM-3-配体信号轴在多种疾病的发病机制中至关重要，包括自身免疫性疾病、感染、癌症、移植排斥和慢性炎症。例如，TIM-3 与 galectin-9 结合可以下调 Th1 反应。此外，Tim-3 表达失调与自身免疫性疾病相关。

生物活性

Immobilized Human Galectin9 at 2 μg/mL can bind recombinant human HAVCR2 with a linear range of 0.3-5 μg/mL.

种属

Human

表达宿主

HEK293 cells

Calculated MW

46.66 kDa

Observed MW

65-75 kDa

标签

C-hFc&His

纯度

> 95% by SDS-PAGE.

内毒素

< 0.1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.

描述

Recombinant Human TIM-3/HAVCR2/CD366 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ser22-Arg200 ) of human TIM-3 (Accession #NP_116171.3) fused with an Fc, 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Galectin9 at 2 μg/mL can bind recombinant human HAVCR2 with a linear range of 0.3-5 μg/mL.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

背景信息

HAVCR2 also known as TIM3(T cell immunoglobulin and mucin domain-3), is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk region. Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail. An alternatively spliced isoform is truncated within the mucin-like stalk.TIM-3 is up‑regulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8+, NK, Treg). Its binding to Galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity . TIM-3 ligation attenuates CD8+ and Th1 cell responses and promotes the activity of Treg and myeloid derived suppressor cells. In addition, dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross-presentation of apoptotic cell antigens. It also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA. TIM-3 interactions with Galectin-9 can alternatively trigger immune stimulatory effects, such as the coactivation of NK cell cytotoxicity.

基因ID

84868

Swiss Prot

Q8TDQ0

别名

HAVCR2;CD366;HAVcr-2;KIM-3;TIM3;TIMD-3;TIMD3;Tim-3; CD366; KIM-3; SPTCL; TIMD3; Tim-3; TIMD-3; HAVcr-2

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活性重组蛋白 Recombinant Human TIM 3 / HAVCR2 / CD366 / KIM-3 / SPTCL Protein

背景信息

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