活性重组蛋白 Recombinant Human Thrombopoietin / THPO / TPO / MGDF / MPLLG Protein

重组血小板生成素可减少放射和化疗引起的血小板减少症，增强骨髓移植后的血小板恢复，并增加干细胞收获物中巨核细胞前体细胞的数量。

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商品分类：重组蛋白　　种属： Human

商品编号： GX004925　　库存： 100支

上市时间： 2024-12-11 16:32:18　　浏览次数： 771次

研究方向

重组血小板生成素可减少放射和化疗引起的血小板减少症，增强骨髓移植后的血小板恢复，并增加干细胞收获物中巨核细胞前体细胞的数量。向动物注射血小板生成素可刺激骨髓巨核细胞的数量、大小和倍性，并使血小板计数增加至十倍。超生理量的血小板生成素/TPO (>100 ng/mL) 能够在体外直接激活血小板聚集。血小板生成素/TPO 对血流下的血小板粘附也有显着影响。低血小板生成素/TPO 浓度 (001-1 ng/mL) 可加速血小板与血管性血友病因子的牢固粘附，并防止在较高流速下脱离。

生物活性

Measured by its ability to activate protein C induced cleavage of the chromogenic substrate, BOC-Asp-Pro Arg-AMC in the presence of thrombin. The specific activity is greater than 500 pmoles/min/ug.

种属

Human

表达宿主

HEK293 cells

Calculated MW

36.31 kDa

Observed MW

70-90 kDa

标签

C-His

纯度

> 97% by SDS-PAGE.

内毒素

< 0.1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.

描述

Recombinant Human Thrombopoietin/THPO Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser22-Gly353) of human Thrombopoietin/THPO (Accession #NP_000451.1) fused with a 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

背景信息

Thrombopoietin (TPO or THPO), also known as myeloproliferative leukemia virus ligand (c-Mpl), is a hematopoietic growth factor belonging to the EPO/TPO family. Megakaryocytopoiesis is the cellular development process that leads to platelet production. TPO is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. TPO is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in TPO gene are the cause of thrombocythemia .

基因ID

7066

Swiss Prot

P40225

别名

THPO;MGDF;MKCSF;ML;MPLLG;THCYT1;TPO

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活性重组蛋白 Recombinant Human Thrombopoietin / THPO / TPO / MGDF / MPLLG Protein

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