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活性重组蛋白 Recombinant Human SMPD1 / ASMase / Asm Protein

SMPD1 是一种多方面的酶,在鞘磷脂动态转化为神经酰胺方面发挥着重要作用,这一过程对于多种细胞功能至关重要。这种转化发生在溶酶体中,其中 SMPD1 在鞘脂代谢中发挥着关键作用。

价 格: 980.00

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商品分类: 重组蛋白  种属: Human

商品编号: GX00208  库存: 100支

上市时间: 2024-12-11 16:00:14  浏览次数: 851次

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研究方向

SMPD1 是一种多方面的酶,在鞘磷脂动态转化为神经酰胺方面发挥着重要作用,这一过程对于多种细胞功能至关重要。这种转化发生在溶酶体中,其中 SMPD1 在鞘脂代谢中发挥着关键作用。值得注意的是,SMPD1 表现出两种不同的酶促形式,这是由单一蛋白质前体的替代运输产生的,其中一种形式靶向内溶酶体区室,另一种形式释放到细胞外。在各种应激条件下,溶酶体胞吐作用成为分泌形式的主要来源,凸显了 SMPD1 对细胞信号的适应性。除了其鞘磷脂酶活性外,SMPD1 对内溶酶体内膜的胆固醇输出也有显着贡献,表明其参与脂质稳态。此外,SMPD1 对特定底物表现出磷脂酶 C 活性,强调了其在脂质代谢中的多功能性。除了脂质加工之外,SMPD1 通过调节神经酰胺的产生来对应激诱导的细胞凋亡进行调节,从而为其在细胞过程中的多功能作用增加了一层复杂性。


生物活性



Measured by its ability to cleave 2-N-Hexadecanoylamino-4- nitrophenylphosphorylcholine (HNPPC). The specific activity is >1,700 pmol/min/μg, as measured underthe described conditions. 



  • 纯度:
    Greater than 95% as determined by SDS-PAGE.
  • 基因名:
  • Uniprot No.:
  • 种属:
    Human
  • 来源:
    293F
  • 分子量:
    71&77&85 kDa
  • 表达区域:
    62-628 aa
  • 氨基酸序列
    LALSDSRVLWAPAEAHPLSPQGHPARLHRIVPRLRDVFGWGNLTCPICKGLFTAINLGLKKEPNVARVGSVAIKLCNLLKIAPPAVCQSIVHLFEDDMVEVWRRSVLSPSEACGLLLGSTCGHWDIFSSWNISLPTVPKPPPKPPSPPAPGAPVSRILFLTDLHWDHDYLEGTDPDCADPLCCRRGSGLPPASRPGAGYWGEYSKCDLPLRTLESLLSGLGPAGPFDMVYWTGDIPAHDVWHQTRQDQLRALTTVTALVRKFLGPVPVYPAVGNHESTPVNSFPPPFIEGNHSSRWLYEAMAKAWEPWLPAEALRTLRIGGFYALSPYPGLRLISLNMNFCSRENFWLLINSTDPAGQLQWLVGELQAAEDRGDKVHIIGHIPPGHCLKSWSWNYYRIVARYENTLAAQFFGHTHVDEFEVFYDEETLSRPLAVAFLAPSATTYIGLNPGYRVYQIDGNYSGSSHVVLDHETYILNLTQANIPGAIPHWQLLYRARETYGLPNTLPTAWHNLVYRMRGDMQLFQTFWFLYHKGHPPSEPCGTPCRLATLCAQLSARADSPALCRHLMPDGSLPEAQSLWPRPLFC
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签:
    C-terminal 10xHis-tagged
  • 产品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 复溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 注意事项:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
别名:
Sphingomyelin phosphodiesterase; Acid sphingomyelinase; ASMase; Smpd1; Asm


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