活性重组蛋白 Recombinant Human CD328 / Siglec-7 / Siglec7 / CDw328 / D-siglec / p75 / AIRM1 / QA79 Protein-武汉瑾萱生物科技有限公司商城首页

研究方向

Siglec-7 蛋白被鉴定为假定的粘附分子，充当唾液酸依赖性细胞结合的介质。它优先结合 α-2,3- 和 α-2,6- 连接的唾液酸，并与二唾液酸神经节苷脂相互作用，例如二唾液酸半乳糖基球苷、二唾液酸乳四糖神经酰胺和二唾液酸 GalNAc 乳四糖神经酰胺。值得注意的是，Siglec-7 的唾液酸识别位点可能由于与同一细胞表面上的唾液酸的顺式相互作用而被掩蔽。在免疫反应中，它充当抑制性受体，在配体结合时诱导酪氨酸磷酸化，并通过其 SH2 结构域招募细胞质磷酸酶，从而阻断信号转导并抑制自然杀伤细胞的细胞毒性。Siglec-7 还可能在造血作用中发挥作用，抑制 CD34+ 细胞前体向骨髓单核细胞谱系的分化，并在体外阻碍白血病骨髓细胞的增殖。此外，它在磷酸化后与 PTPN6/SHP-1 相互作用。

生物活性

Measured by the ability of the immobilized protein to support the adhesion of human red blood cells.The ED50 for this effect is 0.5-2µg/mL.

种属

Human

表达宿主

HEK293 cells

Observed MW

50-60 kDa

标签

C-His

内毒素

<0.1EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

表达区间

19-353 aa

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

背景信息

Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding , followed by varying numbers of Ig-like C2-type domains. Eleven human Siglecs have been cloned and characterized . They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and the Siglec-5 to 11. To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglec-5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system. This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasma tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system. Human Siglec-5 cDNA encodes a 551 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, three Ig-like C2-type domains, a transmembrane region and a cytoplasma tail . Siglec-5 exists as a disulfide-linked homodimer on the cell surface and is expressed on monocytes, neutrophils and B cells . It binds equally well to both alpha 2,3- and alpha 2,6-linked sialic acid .

Swiss Prot

Q9Y286

别名

Adhesion inhibitory receptor molecule 1; adhesion inhibitory receptor molecule 1, siglec-7; AIRM1; AIRM-1; AIRM1QA79 membrane protein; CD328 antigen; CD328; CDw328; D-siglec; p75; p75/AIRM1; QA79; sialic acid binding Ig-like lectin 7; sialic acid binding immunoglobulin-like lectin 7; sialic acid-binding Ig-like lectin 7; Siglec7; Siglec-7

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活性重组蛋白 Recombinant Human CD328 / Siglec-7 / Siglec7 / CDw328 / D-siglec / p75 / AIRM1 / QA79 Protein

背景信息

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