活性重组蛋白 Recombinant Human SCF / Kitl / MGF / SF / SHEP7 / KITLG / DCUA Protein

干细胞因子（SCF）也称为肥大细胞生长因子（MGF）和钢因子（SLF），在配子发生、黑素生成和造血早期阶段发挥重要作用。SCF是一种二聚体分子，通过结合并激活受体酪氨酸激酶c-Kit来发挥其生物学功能。

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商品分类：重组蛋白　　种属： Human

商品编号： GX004911　　库存： 100支

上市时间： 2024-11-20 15:30:23　　浏览次数： 582次

研究方向

生物活性

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 forthis effect is 1.00-8.00 ng/mL.

种属

Human

表达宿主

HEK293 cells

Calculated MW

21.89 kDa

Observed MW

35-38 kDa

标签

C-His

纯度

> 97% by SDS-PAGE.

内毒素

< 0.1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.

描述

Recombinant Human c-Kit ligand/KITLG/SCF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu26-His214) of human SCF/C-kit ligand (Accession #NP_000890.1) fused with a 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

背景信息

The protein is tyrosine-kinase receptor. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis.

基因ID

4254

Swiss Prot

P21583

别名

KITLG;DCUA;DFNA69;FPH2;FPHH;KL-1;Kitl;MGF;SCF;SF;SHEP7

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活性重组蛋白 Recombinant Human SCF / Kitl / MGF / SF / SHEP7 / KITLG / DCUA Protein

背景信息

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