研究方向
AGER（高级糖基化终产物特异性受体）是细胞表面分子免疫球蛋白超家族的成员，是一种跨膜信号转导受体，具有许多配体，包括可以启动和维持免疫反应的警报素。AGER 天然以两种形式存在，即全长膜结合形式和截短（可溶）形式。人类 AGER 是一个高度多态性的基因，其位于 6p21.3 染色体上的基因座有超过 190 个 SNP。AGER 与多种配体和多种信号通路相互作用，例如由高迁移率族盒 1 (HMGB1) 蛋白（AGER 的非经典配体）激活的信号通路。在动脉粥样硬化等某些疾病中，AGER 是急性和慢性血管炎症的介质，尤其是糖尿病的并发症。
生物活性
Immobilized Recombinant human HMGB1 at 2 μg/mL (100 μL/well) can bind Recombinant human AGER with a linear range of 35 ng/mL.
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述
Recombinant Human AGER/RAGE Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Gln24-Ala344) of human AGER/RAGE (Accession #NP_001127.1) fused with an Fc, 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant human HMGB1 at 2 μg/mL (100 μL/well) can bind Recombinant human AGER with a linear range of 15-50 ng/mL.
2.Measured by its binding ability in a functional ELISA. Immobilized Human S100A12 at 2 μg/mL (100 μL/well) can bind recombinant Human AGER/RAGE, the EC50 of Human AGER/RAGE is 27.25 ng/mL.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
背景信息
Receptor for Advanced Glycosylation End Products (RAGE, or AGER) is a member of the immunoglobulin super-family transmembrane proteins, as a signal transduction receptor which binds advanced glycation endproducts, certain members of the S100/calgranulin family of proteins, high mobility group box 1 (HMGB1), advanced oxidation protein products, and amyloid (beta-sheet fibrils). It is a multiligand receptor, and besides AGE, interacts with other molecules implicated in homeostasis, development, and inflammation, and certain diseases, such as atherosclerosis, arthritis, Alzheimer's disease, atherosclerosis and aging associated diseases.
别名
advanced glycosylation end product-specific receptor; AGER; RAGE isoform delta; RAGE isoform sRAGE-delta; RAGE; Receptor for advanced glycosylation end products; receptor for advanced glycosylation end-products; SCARJ1
