研究方向
PVR/CD155，也称为人脊髓灰质炎病毒受体 (PVR)，是免疫球蛋白 (Ig) 样分子亚家族的成员，由 N 端可变结构域、随后的两个恒定结构域、单个跨膜结构域组成区和可变长度的细胞质尾。CD155 结合细胞外基质蛋白玻连蛋白，从而介导细胞与基质的接触。CD155 的胞质尾部与网格蛋白接头复合物的 μ1B 亚基相互作用，导致 CD155 在极化上皮细胞中定向转运。
生物活性
Immobilized Human CD155 at 2 μg/mL (100 μL/well) can bind Human CD96 with a linear range of 0.1-76 ng/mL.
纯度
> 95% by SDS-PAGE；> 95% by HPLC
内毒素
< 0.1 EU/μg of the protein by LAL method
制剂
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
描述
Biotinylated Recombinant Human PVR/CD155 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Trp21-Asn343) of Human PVR/CD155 (Accession #P15151-1) fused with a C-His&Avi tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
背景信息
CD155 is a cell surface adhesion molecule functioning in tumor cell migration, invasion, and metastasis, and not surprisingly, is also designated as a common tumor-associated antigen. CD155 is also recognized by NK cells to induce their cytotoxicity. CD155 is also commonly referred to as the "poliovirus receptor," or PVR.
别名
CD155; HVED; NECL5; Necl-5; nectin-like 5; PVR; PVS; Tage4; PVSFLJ25946; FLJ25946
