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活性重组蛋白 Recombinant Human CEACAM3 / CD66D / CEA / CGM1 Protein

CEACAM3 蛋白是一种关键的粒细胞受体,可协调 CEACAM 结合微生物(包括奈瑟菌属、莫拉氏菌属和嗜血杆菌属等病原体)的高效非调理素依赖性吞噬作用

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商品分类: 重组蛋白  种属: Human

商品编号: GX004837  库存: 100支

上市时间: 2024-11-06 17:39:22  浏览次数: 541次

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研究方向


CEACAM1 蛋白参与多种生物过程。它在胰岛素刺激下被胰岛素受体(INSR)磷酸化,从而促进胰岛素清除并调节肝脏脂肪生成。SF3R基因参与多种生物过程和功能。它通过促进胰岛素的清除和调节肝脏中的脂肪生成来调节胰岛素的作用。在胰岛素刺激下,SF3R 会被胰岛素受体 (INSR) 磷酸化,导致胰岛素内吞作用和降解增加。这导致脂肪酸合成减少。SF3R 还通过与 SHC1 相互作用,在下调细胞增殖中发挥作用,从而减少 SHC1 与 MAPK3/ERK1-MAPK1/ERK2 和磷脂酰肌醇 3-激酶途径的偶联。


种属
Human
表达宿主
HEK293 cells
Calculated MW
17.62 kDa
Observed MW
18-20 kDa
标签
C-His
纯度
> 95% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述
Recombinant Human CEACAM3/CD66d Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Gly155) of human CEACAM3/CD66d (Accession #NP_001806.2) fused with a 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
生物活性
Measured by its binding ability in a functional ELISA. Immobilized Human CEACAM3 at 1μg/mL (100 μL/well) can bind CEACAM3 Mouse mAb with a linear range of 0.1-2.7 ng/mL.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。

背景信息

The protein encodes a member of the family of carcinoembryonic antigen-related cell adhesion molecules (CEACAMs), which are used by several bacterial pathogens to bind and invade host cells. The encoded transmembrane protein directs phagocytosis of several bacterial species that is dependent on the small GTPase Rac. It is thought to serve an important role in controlling human-specific pathogens by the innate immune system. Alternatively spliced transcript variants have been described.

基因ID
Swiss Prot
别名
CEACAM3;CD66D;CEA;CGM1;W264;W282


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