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活性重组蛋白 Recombinant Human CD98 / SLC3A2 / MDU1 / NACAE Protein

CD98 蛋白作为伴侣,有助于功能性转运蛋白异二聚体的生物发生和运输至质膜。

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商品分类: 重组蛋白  种属: Human

商品编号: GX004834  库存: 100支

上市时间: 2024-11-06 16:46:14  浏览次数: 536次

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研究方向


CD98 蛋白作为伴侣,有助于功能性转运蛋白异二聚体的生物发生和运输至质膜。它与各种 SLC7 家族转运蛋白形成异二聚体,包括 SLC7A5、SLC7A6、SLC7A7、SLC7A8、SLC7A10 和 SLC7A11,充当氨基酸反向转运蛋白,其底物特异性由 SLC7A 亚基决定。例如,异二聚体 SLC3A2/SLC7A11 通过介导细胞外阴离子 L-胱氨酸和细胞内 L-谷氨酸的交换而发挥逆向转运蛋白的作用。此外,SLC3A2/SLC7A5 与 LAPTM4B 结合时,会被募集至溶酶体,促进亮氨酸摄取和 mTORC1 激活。CD98 对于各种 SLC7 转运蛋白的质膜定位、稳定性和转运活性至关重要。此外,它在调节整合素相关信号传导方面发挥着关键作用,有助于细胞扩散、迁移和肿瘤进展。在丙型肝炎病毒 (HCV) 感染的情况下,SLC3A2 和 SLC7A5/LAT1 复合物促进病毒进入宿主细胞并增加 L-亮氨酸摄取,从而增强 mTORC1 信号激活并促进 HCV 介导的发病机制。



种属
Human
表达宿主
Baculovirus-Infected Sf9 Cells
Calculated MW
46.85 kDa
Observed MW
45 kDa
标签
No tag
纯度
> 97% by SDS-PAGE.
内毒素
< 1.0 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述
Recombinant Human SLC3A2/CD98 Protein is produced by insect cell-baculovirus expression system. The target protein is expressed with sequence (Arg206-Ala630) of human SLC3A2 (Accession #NP_002385.3).
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
生物活性
Measured by its binding ability in a functional ELISA.Immobilized Human SLC3A2 Protein at 5 μg/mL (100 μL/well) can bind ITGB1 with a linear range of 0.0195-1.216 μg/mL.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。


背景信息

This protein is a member of the solute carrier family and encodes a cell surface, transmembrane protein. The protein exists as the heavy chain of a heterodimer, covalently bound through di-sulfide bonds to one of several possible light chains. The encoded transporter plays a role in regulation of intracellular calcium levels and transports L-type amino acids. Alternatively spliced transcript variants, encoding different isoforms, have been characterized.

基因ID
Swiss Prot
别名
4F2; 4F2HC; 4T2HC; CD98; CD98HC; MDU1; NACAE;SLC3A2;4F2HC;4T2HC;CD98;CD98HC;MDU1;NACAE


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