活性重组蛋白 Recombinant Human B29 / IGB / CD79B Protein

CD79B 蛋白与 CD79A 一起在启动 B 细胞抗原受体复合物 (BCR) 触发的信号转导级联中发挥重要作用。这一关键功能导致复合物的内化，随后运输至晚期内体，以及最终的抗原呈递

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商品分类：重组蛋白　　种属： Human

商品编号： GX004829　　库存： 100支

上市时间： 2024-11-06 16:20:45　　浏览次数： 539次

研究方向

CD79B 蛋白与 CD79A 一起在启动 B 细胞抗原受体复合物 (BCR) 触发的信号转导级联中发挥重要作用。这一关键功能导致复合物的内化，随后运输至晚期内体，以及最终的抗原呈递。CD79B 可能通过招募磷酸化 CD79A 的激酶或通过接合与 CD79A 结合的蛋白质来增强 CD79A 的磷酸化，从而保护其免于去磷酸化。CD79B 与 α 链形成异二聚体，形成二硫键连接的复合物。它是 B 细胞抗原受体复合物的组成部分，其中 α/β 链异二聚体与包含两条重链和两条轻链的抗原特异性膜结合表面免疫球蛋白非共价结合。此外，CD79B 与 LYN 相互作用，进一步促进其在 B 细胞信号传导中的调节作用。

种属

Human

表达宿主

HEK293 cells

Calculated MW

16.05 kDa

Observed MW

25-38 kDa

标签

C-His

纯度

> 90% by SDS-PAGE.

内毒素

< 1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

描述

Recombinant Human B29/CD79B Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala29-Asp159) of human CD79B (Accession #NP_000617.1) fused with a 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured by its binding ability in a functional ELISA.Immobilized Human CD79B at 0.5 μg/mL (100 μL/well) can bind CD79B Rabbit mAb with a linear range of 0.1-1.2 ng/mL.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

背景信息

B-cell antigen receptor complex-associated protein beta chain (CD79b) is also known as B-cell-specific glycoprotein B29, Ig-beta,Immunoglobulin-associated B29 protein, B29 and IGB, which is a single-pass type I membrane protein containing one Ig-like V-type ( immunoglobulin-like ) domain and one ITAM domain.CD79b is required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR).CD79b can enhance phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation. Defects in CD79b are the cause of agammaglobulinemia type 6 (AGM6) that is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development.

基因ID

974

Swiss Prot

P40259

别名

AGM6; B29; IGB;CD79B;B29;IGB

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活性重组蛋白 Recombinant Human B29 / IGB / CD79B Protein

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