活性重组蛋白 Recombinant Human Placenta growth factor/PlGF-131/PlGF-1 Protein-武汉瑾萱生物科技有限公司商城首页

研究方向

PLGF-2 蛋白是一种在血管生成和内皮细胞生长中具有显着活性的生长因子，在刺激这些细胞的增殖和迁移中发挥着至关重要的作用。通过与 FLT1/VEGFR-1 受体结合，PLGF-2 协调血管生成过程并有助于调节血管生长。值得注意的是，异构体 PlGF-2 表现出额外的结合能力，以肝素依赖性方式与 NRP1/neuropilin-1 和 NRP2/neuropilin-2 形成相互作用。除了其血管生成功能外，PLGF-2 还促进肿瘤生长，表明其参与与癌症相关的病理性血管生成。在结构上，PLGF-2以二硫键连接的反向平行同二聚体的形式存在，并且可以进一步表现为与VEGFA/VEGF的异二聚体。同工型 PlGF-3 作为同二聚体和单体的存在增加了 PLGF 蛋白的复杂性，突出了它们在调节血管过程和肿瘤发生中的不同作用。

种属

Human

表达宿主

HEK293 cells

Calculated MW

40.68 kDa

Observed MW

55-60 kDa

标签

C-hFc

内毒素

< 0.1 EU/μg

纯度

>95%

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

描述

Recombinant Human Placenta growth factor/PlGF-131/PlGF-1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Leu19-Arg149) fused with a hFc tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured by its binding ability in a functional ELISA. Immobilized human VEGFR1/Flt-1 (Catalog: RP01188) at 2 μg/mL (100 μL/well) can bind Human Placenta growth factor/PLGF-131 (Catalog: RP02896) with a linear range of 0.17-41.1 ng/mL.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

背景信息

Placenta growth factor (PlGF) is a member of the PDGF/VEGF family of growth factors that share a conserved pattern of eight cysteines (1, 2). Alternative splicing results in at least three human mature PlGF forms containing 131 (PlGF-1), 152 (PlGF-2), and 203 (PlGF-3) amino acids (aa) respectively (1, 2). Only PlGF-2 contains a highly basic heparin-binding 21 aa insert at the C-terminus (1). Human PlGF-1 shares 56%, 55%, 74% and 95% aa identity with the comparable isoform of mouse, rat, canine, and equine PlGF, respectively. PlGF is mainly found as variably glycosylated, secreted, 55-60 kDa disulfide linked homodimers (3). Mammalian cells expressing PlGF include villous trophoblasts, decidual cells, erythroblasts, keratinocytes, and some endothelial cells (1, 4-6). Circulating PlGF increases during pregnancy, reaching a peak in mid-gestation; this increase is attenuated in preeclampsia (7). However, deletion of PlGF in the mouse does not affect development or reproduction. Postnatally, mice lacking PlGF show impaired angiogenesis in response to ischemia (8).

基因ID

5228

Swiss Prot

P49763-2

别名

D12S1900; PGFL; PIGF; PLGF; PlGF-2; SHGC-10760;PGF;PGFL;PIGF;PLGF;PlGF-2;SHGC-10760

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活性重组蛋白 Recombinant Human Placenta growth factor/PlGF-131/PlGF-1 Protein

背景信息

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