研究方向
骨膜素蛋白作为多方面的调节剂出现，证明其能够诱导细胞附着和扩散，同时积极参与细胞粘附。此外，Periostin 还能增强 BMP1 与结缔组织内纤连蛋白基质的结合，从而促进赖氨酰氧化酶 LOX 的蛋白水解激活。这种多功能蛋白质以同二聚体形式存在，强调了其结构意义。值得注意的是，Periostin 与 BMP1 和纤连蛋白相互作用，强调其参与有助于细胞粘附、组织结构和细胞外基质调节的关键分子过程。 Periostin 及其结合伙伴之间复杂的相互作用使其成为细胞和组织功能动态调节的关键参与者。
标签
C-His
纯度
>95%
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
描述
Recombinant Human Periostin/PN/POSTN Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asn22-Gln836) of human Periostin/PN/POSTN (Accession #NP_006466.2) fused with a 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. Immobilized Recombinant Human Periostin/OSF‑2 at 10 µg/mL (100 µL/well) induces >19% cell adhesion.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
背景信息
Periostin ( POSTN ), also known as OSF2 (osteoblast specific factor 2), is a heterofunctional secreted extracellular matrix (ECM) protein comprised of four fasciclin domains that promotes cellular adhesion and movement, as well as collagen fibrillogenesis.Postn is frequently overexpressed in various types of human cancers, stimulating metastatic growth by promoting cancer cell survival, invasion and angiogenesis, and can be a useful marker to predict the behavior of cancer.
别名
PN; OSF2; OSF-2; PDLPOSTN;POSTN
