活性重组蛋白 Recombinant Human CD32A / IGFR2 / FCGR2A / CDw32 / FCGR2 Protein (H167R)-武汉瑾萱生物科技有限公司商城首页

研究方向

Fc γ RIIA/CD32a 蛋白通过特异性结合免疫球蛋白 γ 的 Fc 区而发挥关键作用，充当低亲和力受体。通过与 IgG 相互作用，Fc gamma RIIA/CD32a 启动针对病原体和可溶性抗原的细胞反应，说明其在免疫调节中的重要作用。值得注意的是，该蛋白质促进调理抗原的吞噬作用，进一步促进免疫防御机制。此外，Fc gamma RIIA/CD32a 与 IGHG1、INPP5D/SHIP1、INPPL1/SHIP2、APCS、FGR 和 HCK 相互作用，表明其参与复杂的信号传导途径及其细胞功能的调节。这些多方面的相互作用强调了 Fc gamma RIIA/CD32a 在协调不同免疫反应中的重要性。

生物活性

Immobilized recombinant human CD32a at 1 μg/mL (100 μL/well) can bind biotinylated human IgG1 with a linearrange of 30-250 ng/mL.

种属

Human

表达宿主

HEK293 cells

描述

Recombinant Human Fc-gamma RIIa/CD32a(H167R) Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala36-Ile218 (R167)) of human Fc gamma RIIA/CD32a (Accession #NP_001129691.1) fused with a 6×His tag at the C-terminus.

标签

C-His

纯度

> 97% by SDS-PAGE.

内毒素

< 0.1 EU/μg of the protein by LAL method.

生物活性

Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD32a at 1 μg/mL (100 μL/well) can bind biotinylated human IgG1 with a linear range of 30-250 ng/mL.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.

储存

Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

研究方向

背景信息

The member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.

基因ID

2212

Swiss Prot

P12318

别名

FCGR2A;CD32;CD32A;CDw32;FCG2;FCGR2;FCGR2A1;FcGR;IGFR2

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活性重组蛋白 Recombinant Human CD32A / IGFR2 / FCGR2A / CDw32 / FCGR2 Protein (H167R)

研究方向

背景信息

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