研究方向
Biglycan 蛋白作为胶原纤维组装的潜在参与者而出现，表明它在组织和构建胶原蛋白网络的复杂过程中发挥着作用。它参与胶原纤维组装意味着在细胞外基质的形成和维持中具有至关重要的功能。作为结缔组织的重要组成部分，Biglycan 可能有助于胶原纤维的稳定性和完整性。阐明 Biglycan 参与胶原蛋白组装的具体机制可以为其在组织发育、修复和体内平衡中的作用提供有价值的见解。对 Biglycan 功能的进一步探索可能会加深我们对其在结缔组织生物学中的影响及其在各种生理环境中的潜在意义的理解。
描述
Recombinant Human Biglycan/BGN Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Asp38-Lys368) of human Biglycan/BGN (Accession #NP_001702.1) fused with a 6×His tag at the C-terminus.
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
储存
Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
背景信息
Biglycan, also known as PG I, is a secreted chondroitin/dermatan sulfate proteoglycan in the small leucine-rich proteoglycan (SLRP) family.The protein is plays a role in bone growth, muscle development and regeneration, and collagen fibril assembly in multiple tissues. This protein may also regulate inflammation and innate immunity. Additionally, It may contribute to atherosclerosis and aortic valve stenosis in human patients.
别名
BGN;DSPG1;MRLS;PG-S1;PGI;SEMDX;SLRR1A;biglycan
