研究方向
CD14 编码一种在先天免疫反应中至关重要的蛋白质，并在单核细胞/巨噬细胞中显着表达。作为辅助受体，它结合各种微生物和真菌分子，包括脂多糖（LPS）。LPS 结合蛋白 (LBP) 增强了该蛋白的 LPS 结合活性，促进与 TLR4-MD-2 共受体复合物的结合。CD14 以两种形式存在：可溶性形式和由糖基磷脂酰肌醇锚定物锚定在细胞表面的形式。它在乳腺和结肠等多种组织中广泛表达，突显了其在免疫相关过程中的关键作用。
纯度
> 97 % as determined by SDS-PAGE
内毒素
< 0.1 EU per μg of the protein as determined by the LAL method
生物活性
Measured by its ability to enhance LPS-induced IL-6 secretion by mouse splenocytes. The ED50 for this effect is 0.4-2.4 µg/mL.
蛋白构建
A DNA sequence encoding the extracellular domain (Met 1-Pro 345) of mouse CD14 (NP_033971.1) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
分子量
The recombinant mouse CD14/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 578 amino acids and has a predicted molecular mass of 63.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CD14/Fc monomer is approximately 85-95 kDa due to glycosylation.
缓冲液
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
