研究方向
gp130/IL6ST 是一种信号转导分子，是多种细胞因子（包括 IL6、LIF、OSM、CNTF、IL11、CTF1 和 BSF3）受体系统的重要组成部分，促进信号传递。IL6 与 IL6R 结合后，IL6ST 发生同二聚化，形成高亲和力受体复合物，激活 JAK-MAPK 和 JAK-STAT3 信号通路。这种激活导致 IL6ST 酪氨酸残基磷酸化，随后激活 STAT3。同时，IL6 信号传导诱导细胞因子受体信号传导抑制剂 SOCS1 和 SOCS3 的表达，建立负反馈回路来调节 JAK 活性（通过相似性）。此外，IL6ST 通过激活 yes 相关蛋白 1 (YAP) 和 NOTCH 通路（通过相似性），在独立于 STAT3 的控制炎症诱导的上皮再生中发挥作用。IL6ST 作为神经保护肽人肽的受体，与 IL27RA/WSX1 和 CNTFR 结合，介导对免疫反应、造血、疼痛控制、骨代谢和胚胎发育至关重要的信号（通过相似性）。此外，IL6ST 对于运动和感觉神经元的存活和分化、伤害感受神经元中 TRPA1 的表达以及成骨细胞谱系中 PTH1R 表达的维持至关重要（通过相似性）。它与可溶性 IL6:sIL6R 复合物（称为 hyper-IL6）结合，抑制 IL6 反式信号传导并通过 IL11R 阻断 IL11 簇信号传导。
纯度
> 95 % as determined by SDS-PAGE.
内毒素
< 0.1 EU per μg of the protein as determined by the LAL method
生物活性
Measured by its ability to inhibit the IL-6Rα enhancement of IL-6 activity on M1 mouse myeloid leukemia cell. The ED50 for this effect is typically 0.2-0.8 μg/mL in the presence of 50 ng/mL recombinant human IL-6sR and 100 ng/mL recombinant human IL-6.
蛋白构建
A DNA sequence encoding the extracellular domain (Met 1-Ile 618) of human IL6ST (NP_002175.2) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
分子量
The recombinant human IL6ST/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 844 amino acids and has a predicted molecular mass of 96 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh IL6ST/Fc monomer is approximately 125-140 kDa due to glycosylation.
缓冲液
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
别名
CD130 Protein, Human; CDW130 Protein, Human; GP130 Protein, Human; IL-6RB Protein, Human
