研究方向
CD276/B7-H3 蛋白被认为在调节 T 细胞介导的免疫反应中发挥多方面的作用，通过抑制自然杀伤介导的细胞裂解，潜在地充当肿瘤细胞的保护因子，并作为肿瘤细胞的标记物。神经母细胞瘤细胞的检测。此外，CD276/B7-H3 可能参与急性和慢性移植排斥的发生，有助于调节粘膜表面的淋巴细胞活性。值得注意的是，它在整个怀孕期间为胎盘和胎儿提供适宜的免疫环境方面发挥着至关重要的作用。CD276/B7-H3 的亚型 1 和亚型 2 在调节 CD4 T 细胞反应的能力上似乎都是多余的，亚型 2 被证明可以增强细胞毒性 T 细胞的诱导，并在 T 存在的情况下选择性刺激干扰素 γ 的产生。-细胞受体信号传导。与 TREML2 的相互作用被确定为增强 T 细胞激活，突显了 CD276/B7-H3 在免疫调节和细胞反应中可能发挥的不同作用。
纯度
> 95 % as determined by SDS-PAGE.
内毒素
< 0.1 EU per μg protein as determined by the LAL method.
生物活性
Immobilized Human B7-H3 at 2 μg/mL (100μL/well) can bind Anti-Human B7-H3 Antibody with a linear range of 1-4 ng/mL.
蛋白构建
A DNA sequence encoding the human CD276 (Q5ZPR3-2, isoform 2) (Met1-Thr243) was expressed with a polyhistidine tag at the C-terminus.
分子量
The recombinant human CD276 consists of 226 amino acids and predicts a molecular mass of 24.52 kDa. It migrates as an approximately 38-48 kDa band in SDS-PAGE under reducing conditions.
缓冲液
Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
