纯度
> 95 % as determined by SDS-PAGE.
内毒素
< 0.1 EU per μg protein as determined by the LAL method.
生物活性
Measured by its ability to inhibit Anti-CD3-induced proliferation of jurkat. The ED50 for this effect is 20-100 ng/mL.
蛋白构建
A DNA sequence encoding the human BTN3A1 (NP_919423.1) (Met1-Gly254) was expressed with the Fc region of human IgG1 at the C-terminus.
NP No.
NP_919423.1
表达宿主
HEK293 Cells
种属
Human
预测 N 端
Gln 30
分子量
The recombinant human BTN3A1 consists 463 amino acids and predicts a molecular mass of 50.9 kDa.
缓冲液
Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of COA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
联系提供实测SDS-Page和活性验证数据。
BTN3A1 Protein, Human, Recombinant (ECD, hFc Tag): 别称
BT3.1 Protein, Human; BTF5 Protein, Human; BTN3.1 Protein, Human; CD277 Protein, Human
BTN3A1 背景信息
BTN3A1 has the structure of a type I receptor of the Ig superfamily and is part of a family of seven BTN receptors encoded by genes in the MHC. BTN molecules are composed of two Ig domains (IgV, IgC2), a single transmembrane domain, and a large carboxyl-terminal domain termed B3.2 (or PRYSPRY) located in the cell cytoplasm. There are three human BTN3A loci, BTN3A1, BTN3A2, and BTN3A3, and clear orthologs of BTN3A molecules, now called CD277, are absent from the mouse genome. Despite its similarity to B7 molecules, BTN3A1 was proposed to act not as a coreceptor or costimulatory molecule, but rather to directly present pAg to the γδ TCR in a manner analogous to MHC-restricted peptide presentation. However, this model of BTN3A1 function has been challenged by conflicting data, which show pAg binding to a positively charged pocket in the cytosolic B3.2 domain, and that BTN3A1 does not directly engage the γδ TCR. This contradictory picture has emerged as a result of the complexity of the system and in particular by the use of endogenous and exogenous routes of Ag delivery in in vitro assays.
Cancer Immunotherapy
Immune Checkpoint
Immunotherapy
Targeted Therapy
全称
butyrophilin subfamily 3 member A1
