研究方向
IL-36α是IL-36家族的一个亚型，属于IL-1超家族。IL-36 α 在单核细胞、T/B 淋巴细胞、脾脏、骨髓扁桃体、淋巴结和皮肤中表达。IL-36α的氨基酸序列在不同物种中有所不同。人 IL-36 α 与小鼠的氨基酸序列同一性<55%。
IL-36α与IL-36R结合并激活NF-κB和MAPK信号通路，从而介导炎症反应。但激活需要中性粒细胞颗粒衍生的蛋白酶（例如组织蛋白酶 G、弹性蛋白酶和蛋白酶-3）进行 N 末端裂解。IL-36 α 还可以结合 IL-1Rrp2 并募集 IL-1RAcP。IL-36 α 通过 IL-36R/IL-1RAcP 激活 MAPK、Erk1/2 和 JNK。感染后IL-36α显着升高。IL-36 α 在调节巨噬细胞的抗菌功能中发挥着关键作用，这是预防小鼠败血症所必需的。IL-36 α是真菌性角膜炎免疫反应中的促炎因子，可促进角膜炎症。
IL-36 α是与一些感染性和免疫性相关的促炎细胞因子疾病。IL-36 α 通过激活 NF-κB 和 MAPK 信号通路介导炎症反应。
纯度
> 97 % as determined by SDS-PAGE
内毒素
Please contact us for more information.
生物活性
Yes
蛋白构建
A DNA sequence encoding the mouse IL1F6 (Q9JLA2-1) (Met 1-His 160) was expressed and purified.
Uniprot No.
Q9JLA2-1
表达宿主
E. coli
种属
Mouse
预测 N 端
Met 1
分子量
The recombinant mouse IL1F6 consisting of 160 amino acids and migrates with an apparent molecular mass of 18 kDa as predicted.
缓冲液
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
IL36 alpha/IL-1F6 Protein, Mouse, Recombinant:
别称
FIL1 Protein, Mouse; FIL1(EPSILON) Protein, Mouse; FIL1E Protein, Mouse; IL-1F6 Protein, Mouse; IL1(EPSILON) Protein, Mouse; IL1F6 Protein, Mouse
IL36 alpha/IL-1F6
背景信息
Interleukin-1 family member 6 (IL-1F6), also known as interleukin 36, alpha (IL36A), is a pro-inflammatory cytokine that plays an important role in innate and adaptive immunity. IL-1F6 activates MAPK and NF-kB pathways and is produced by many different cells. This cytokine is a family member of interleukin-1 (IL-1) and plays an important role in the pathophysiology of several diseases. It has been reported that IL-1F6 and IL-1F8, in addition to IL-1F9, activate the pathway leading to NF-kappaB in an IL-1Rrp2-dependent manner in Jurkat cells as well as in multiple other human and mouse cell lines. Activation of the pathway leading to NF-kappaB by IL-1F6 and IL-1F8 follows a similar time course to activation by IL-1beta, suggesting that signaling by the novel family members occurs through a direct mechanism. In a mammary epithelial cell line, NCI/ADR-RES, which naturally expresses IL-1Rrp2, all three cytokines signal without further receptor transfection. IL-1Rrp2 antibodies block activation of the pathway leading to NF-kappaB by IL-1F6, IL-1F8, and IL-1F9 in both Jurkat and NCI/ADR-RES cells. Thus IL-1F6, IL-1F8, and IL-1F9 signal through IL-1Rrp2 and IL-1RAcP.
全称
interleukin 36, alpha
