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| 种属 | Mouse |
|---|---|
| 表达宿主 | E. coli |
| 描述 | Recombinant Mouse TNFSF11/RANK L/TRANCE Protein is produced by E. coli expression system. The target protein is expressed with sequence (Arg156-Asp316) of mouse TNFSF11/RANK L/TRANCE (Accession #O35235). |
| 标签 | No tag |
| 纯度 | > 95% by SDS-PAGE. |
| 内毒素 | < 1 EU/μg of the protein by LAL method. |
| 生物活性 | Measured by its ability to induce mouse bone marrow cells to differentiate into Osteoclasts. |
| 制剂 | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| 储存 |
Store the lyophilized protein at -20°C to -80°C for 12 months. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月； 复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。 |
| 复溶 | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。 |
Mouse tumor necrosis factor ligand superfamily member 11(Tnfsf11) is a member of the tumor necrosis factor(TNF) cytokine family. Tnfsf11 is widely expressed in cells including T cells and T cell rich organs, such asthymus and lymph nodes. This cytokine can bind to TNFRSF11B/OPG andTNFRSF11A/RANK. Tnfsf11 is involvedin a number of fundamental biological processes such as acting as regulator of interactions between T-cells anddendritic cells, the regulation of the T-cell-dependent immune response and enhancing bone-resorption inhumoral hypercalcemia of malignancy. It augments the ability of dendritic cells to stimulate naive T-cellproliferation.
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